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https://elib.bsu.by/handle/123456789/291295
Заглавие документа: | U-Net-based deep learning for tracking and quantitative analysis of intracellular vesicles in time-lapse microscopy images |
Авторы: | Liu, Zhichao Zhang, Heng Jin, Luhong Chen, Jincheng Nedzved, Alexander Ablameyko, Sergey Ma, Qing Yu, Jiahui Xu, Yingke |
Тема: | ЭБ БГУ::ЕСТЕСТВЕННЫЕ И ТОЧНЫЕ НАУКИ::Математика ЭБ БГУ::ЕСТЕСТВЕННЫЕ И ТОЧНЫЕ НАУКИ::Кибернетика |
Дата публикации: | 2022 |
Издатель: | World Scientific |
Библиографическое описание источника: | J Innovative Opt Health Sci 2022;15(5). |
Аннотация: | Fluorescence microscopy has become an essential tool for biologists, to visualize the dynamics of intracellular structures with specific labeling. Quantitatively measuring the dynamics of moving objects inside the cell is pivotal for understanding of the underlying regulatory mechanism. Protein-containing vesicles are involved in various biological processes such as material transportation, organelle interaction, and hormonal regulation, whose dynamic characteristics are significant to disease diagnosis and drug screening. Although some algorithms have been developed for vesicle tracking, most of them have limited performance when dealing with images with low resolution, poor signal-to-noise ratio (SNR) and complicated motion. Here, we proposed a novel deep learning-based method for intracellular vesicle tracking. We trained the U-Net for vesicle localization and motion classification, with demonstrates great performance in both simulated datasets and real biological samples. By combination with fan-shaped tracker (FsT) we have previously developed, this hybrid new algorithm significantly improved the performance of particle tracking with the function of subsequently automated vesicle motion classification. Furthermore, its performance was further demonstrated in analyzing with vesicle dynamics in different temperature, which achieved reasonable outcomes. Thus, we anticipate that this novel method would have vast applications in analyzing the vesicle dynamics in living cells. |
URI документа: | https://elib.bsu.by/handle/123456789/291295 |
DOI документа: | 10.1142/S1793545822500316 |
Scopus идентификатор документа: | 85135407011 |
Финансовая поддержка: | the National Key Research and Development Program of China (2021YFF0700305 and 2018YFE0119000), the National Natural Science Foundation of China (22104129 and 62105288), Zhejiang Province Science and Technology Research Plan (2022C03014), the Fundamental Research Funds for the Central Universities (2021XZZX022) and Alibaba Cloud |
Лицензия: | info:eu-repo/semantics/openAccess |
Располагается в коллекциях: | Статьи факультета прикладной математики и информатики |
Полный текст документа:
Файл | Описание | Размер | Формат | |
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4438-Текст статьи-42560-1-10-20220510.pdf | 606,44 kB | Adobe PDF | Открыть |
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