Endothelial progenitor cells and plaque burden in stented coronary artery segments: An optical coherence tomography study six months after elective PCI

Abstract

Background: Endothelial progenitor cells (EPC) are involved in neovascularization and endothelial integrity. They might be protective in atherosclerosis. Optical coherence tomography (OCT) is a precise intracoronary imaging modality that allows assessment of subintimal plaque development. We evaluated the influence of EPC on coronary plaque burden in stable disease and implemented a novel computational plaque analysis algorithm using OCT. Methods: Forty-three patients (69.8% males, 69.6±7.7years) were investigated by OCT during re-angiography 6months after elective stent implantation. Different subpopulations of EPCs were identified by flow cytometry according to their co-expression of antigens (CD34+, CD133+, kinase domain receptor, KDR+). An algorithm was applied to calculate the underlying total plaque burden of the stented segments from OCT images. Plaque morphology was assessed according to international consensus in OCT imaging. Results: A cumulative sub-strut plaque volume of 10.87±12.7mm3 and a sub-stent plaque area of 16.23±17.0mm2 were found within the stented vessel segments with no significant differences between different stent types. All EPC subpopulations (mean of EPC levels: CD34+/CD133+: 2.66±2.0%; CD34+/KDR+: 7.50±5.0%; CD34+/CD133+/KDR+: 1.12±1.0%) inversely correlated with the identified underlying total plaque volume and plaque area (p≤0.012). Conclusions: This novel analysis algorithm allows for the first time comprehensive quantification of coronary plaque burden by OCT and illustration as spread out vessel charts. Increased EPC levels are associated with less sub-stent coronary plaque burden which adds to previous findings of their protective role in atherosclerosis.