Еhe effect of hypoxia on photocytotoxicity of tics tricarbocyanine dye in vitro

Abstract

Aim: To evaluate the effect of cell oxygenation onphotocytotoxicity of a novel tricarbocyanine indolenine dye covalently bound to glucose(TICS). Methods: HeLa cells were incubated with 5 μM TICS, 2 h later irradiated by laser at 740 nm with a light dose of 10 J/cm2, delivered at a power density of 10, 20, 25 or 30 mW/cm2, in air or in argon atmosphere, and then scored for viability. Results: The photocytotoxicity of TICS increased dramatically as the power density was reduced. Under hypoxia TICS-photosen-sitized cell death was determined but its value was lowered, compared to photoirradiation in the air. Conclusion: Photosensitizing effect of TICS is only partially dependent on the oxygenation of tumor cells.Key Words:photosensitizers, cyanine dyes, HeLa cells, photodynamic therapy, hypoxia.