Plasmon-Enhanced WGM-based Biosensors for Molecule Detection

Abstract

Novel technique based on optical resonance on microring structures, plasmon resonance and identification tools has been developed. Experimental data on detection and identification of variety of biochemical agents, such as proteins, microelements, antibiotics of different generation etc. in both single and multicomponent solutions analyzed on the light scattering of whispering gallery mode optical resonance are represented. Multiplexing on parameters and components has been realized using developed fluidic sensor cell with fixed in adhesive layer dielectric microspheres and data processing. To improve sensitivity of microring structures microspheres had been treated previously by gold nanoparticle solution. Another technique included usage of thin film gold layers deposited on the substrate below adhesive. Both biomolecule and nanoparticle injections cause considerable changes of optical resonance spectra. Plasmonic gold layers under optimized thickness also improve parameters of optical resonance spectra. Biochemical component identification has been performed by developed network analysis techniques. Proposed approach is demonstrated to be applicable both for single agent and for multicomponent biochemical analysis. So advantages of plasmon enhancing optical microcavity resonance with multiparameter identification tools have been used for development of a new platform for ulta-sensitive label-free biomedical sensor.