Obtaining Primary Cell Cultures and Developing Primers for Analysing the Biological Effects of Phenazines

Abstract

The article presents the results of experimental work on obtaining primary cultures of eukaryotic cells of the Rattus norvegicus Wistar line from various tissues. The results of studies on the selection of marker genes for analysing the biological effect of phenazines on eukaryotic cells are presented, as well as bioinformatic studies on the development of primers for real-time PCR in cells before and after treatment with phenazines. It was possible to generate lines of primary cultures of liver, skin, bone marrow and spinal cord cells of the R. norvegicus Wistar line. In addition, primers for the most important selected marker genes of rat and human were developed for the subsequent analysis of the biological effects of phenazines.