Rod and spherical selenium nanoparticles: Physicochemical properties and effects on red blood cells and neutrophils

Abstract

Abstract The influence of selenium (Se) nanoparticles in the form of rods (SeNrs) and spheres (SeSps), synthesized by laser ablation, on the structural and functional properties of human blood erythrocytes and neutrophils was studied for anticancer activity in vitro. SeNrs and SeSps do not have cytotoxicity towards neutrophils and do not cause hemolysis. The elastic modulus and resistance of erythrocytes to HOCl-induced hemolysis increased after binding of Se nanoparticles to the plasma membrane. The interaction of Se nanoparticles with neutrophils is accompanied by their actin-dependent macropinocytosis, triggering intracellular signaling processes leading to the assembly and activation of NADPH oxidase. Comparative analysis of the effects of SeNrs and SeSps on cells showed that they have similar effects. This may be due to the fact that SeNrs interact with the cell surface with their end faces, and, therefore, have the same initial contact with the plasma membrane as SeSps. However, SeSps and SeNrs showed chronic cytotoxicity after 48 h incubation, indicating the need to find ways to reduce their toxicity further. Further use of Se nanoparticles in anisotropic form in biomedical research for the development of therapeutic agents seems promising.

Keywords: Cytotoxicity; Erythrocytes; Granulocytes; Hemolysis; Nanorods; Nanospheres; ROS.