New application of the commercially available dye celestine blue B as a sensitive and selective fluorescent “turn-on” probe for endogenous detection of HOCl and reactive halogenated species

Reut, V. E.; Kozlov, S. O.; Kudryavtsev, I. V.; Grudinina, N. A.; Kostevich, V. A.; Gorbunov, N. P.; Grigorieva, D. V.; Kalvinkovskaya, J. A.; Bushuk, S. B.; Varfolomeeva, E. Y.; Fedorova, N. D.; Gorudko, I. V.; Panasenko, O. M.; Vasilyev, V. B.; Sokolov, A. V.

doi:10.3390/antiox11091719

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Please use this identifier to cite or link to this item: https://elib.bsu.by/handle/123456789/286193

Title:	New application of the commercially available dye celestine blue B as a sensitive and selective fluorescent “turn-on” probe for endogenous detection of HOCl and reactive halogenated species
Authors:	Reut, V. E. Kozlov, S. O. Kudryavtsev, I. V. Grudinina, N. A. Kostevich, V. A. Gorbunov, N. P. Grigorieva, D. V. Kalvinkovskaya, J. A. Bushuk, S. B. Varfolomeeva, E. Y. Fedorova, N. D. Gorudko, I. V. Panasenko, O. M. Vasilyev, V. B. Sokolov, A. V.
Keywords:	ЭБ БГУ::ЕСТЕСТВЕННЫЕ И ТОЧНЫЕ НАУКИ::Физика
Issue Date:	2022
Citation:	Antioxidants. – 2022. – Vol. 11, № 9. – P. 1719:1-1719:34.
Abstract:	Abstract: Hypochlorous acid (HOCl) derived from hydrogen peroxide and chloride anion by myeloperoxidase (MPO) plays a significant role in physiological and pathological processes. Herein we report a phenoxazine-based fluorescent probe Celestine Blue B (CB) that is applicable for HOCl detection in living cells and for assaying the chlorinating activity of MPO. A remarkable selectivity and sensitivity (limit of detection is 32 nM), along with a rapid “turn-on” response of CB to HOCl was demonstrated. Furthermore, the probe was able to detect endogenous HOCl and reactive halogenated species by fluorescence spectroscopy, confocal microscopy, and flow cytometry techniques. Hence, CB is a promising tool for investigating the role of HOCl in health and disease and for screening the drugs capable of regulating MPO activity. Keywords: hypochlorous acid, myeloperoxidase, neutrophils, fluorescent probes, reactive halogen species, phenoxazine
URI:	https://elib.bsu.by/handle/123456789/286193
DOI:	10.3390/antiox11091719
Licence:	info:eu-repo/semantics/openAccess
Appears in Collections:	Кафедра биофизики (статьи)

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