Глюкозорегулируемая эктопическая экспрессия гена одноцепочечного инсулина в мезенхимальных стволовых клетках человека

Abstract

The technology of stem cells as well as genetic engineering methods being combined open new perspectives for therapy of diabetes mellitus. We have developed a bicistronic lentiviral transfer vector pHR-Pegr1-SCI, carrying single-chain insulin gene and enhanced green fluorescent protein eGFP reporter gene under control of glucose-responsive promoter of human egr1 gene. Recombinant pHR-Pegr1-SCI based lentiviruses were used for transduction of human bone marrowderived mesenchymal stem cells. Under experimental conditions we observed induced 4,7-fold growth in insulin gene expression 15 minutes after glucose concentration being elevated from 3 to 20 mM. Meanwhile after additional 15 minutes the expression level spontaneously decreased up to near baseline level and didn’t change significantly during the subsequent 30 minutes