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https://elib.bsu.by/handle/123456789/204460| Title: | Получение очищенных метаболитов пути утилизации пектиновых веществ |
| Authors: | Мямин, Владислав Евгеньевич Песнякевич, Александр Георгиевич Прокулевич, Владимир Антонович |
| Keywords: | ЭБ БГУ::ЕСТЕСТВЕННЫЕ И ТОЧНЫЕ НАУКИ::Биология |
| Issue Date: | 2003 |
| Publisher: | Минск : БГУ |
| Citation: | Вестник Белорусского государственного университета. Сер. 2, Химия. Биология. География. – 2003. – № 1. – С. 47-51. |
| Abstract: | The molecular study of phytopatogenic bacteria requires metabolites of the pectin utilization pathway. 2,5-Dlketo-3-deoxygluconate (DKII) was purified through Ion change column chromatography from the cultural liquid of a kduD mutant of Erwinia carotovora subsp. atroseptica (strain P177). 2-Keto-3-deoxygluconate was prepared fermentativeiy by treatment of cultural liquid of P177 with 2,5-dlketo-3-deoxygluconate dehydrogenase (crude cell extract of wild type Erwinia carotovora subsp. atroseptica 3-2 was used as enzyme preparation) and then purified the same way as DKII. Metabolites prepared by this method are useful for Investigation of pathogenicity and virulence factors of phytopathogenic bacteria. |
| URI: | http://elib.bsu.by/handle/123456789/204460 |
| ISSN: | 0372-5340 |
| Licence: | info:eu-repo/semantics/openAccess |
| Appears in Collections: | 2003, №1 (февраль) |
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