Анализ нуклеотидной последовательности гена NAHG у природных бактерий Pseudomonas Fluorescens NL61

Abstract

The nucleotide sequence of gene nahG (size 813 bp) which is widespread among natural bacteria Pseudomonas circulating in Belarus was determined in the present paper. Due to the presence of the nucleotide sequences of genes nahG type A88 (presumably localized in the plasmid pA88 of IncP-9 group in bacteria P. fluorescens A88, its sequence is determined and have size 813 bp) and NAH7 (localized in the plasmid NAH7 of IncP-9 group in bacteria Pseudomonas putida NCIB (complete open reading frame is 1305 bp)) made it possible to determine the investigated determinants relevant to type A88. It differs from the known sequence of this type by following nucleotide substitution (it was substitution of thymine to cytosine in 651 position), which led to an additional restriction site for the enzyme RsaI. Identified feature of the gene nahG organization in bacteria P. fluorescens NL61 can be a reliable diagnostic marker in detection of this type of determinants in the analysis of natural and collection naphthalene-utilizing bacteria of the Pseudomonas genus.