Разработка технологии отбора и анализа трансгенных растений картофеля с геном gox penicillium funiculosum

Abstract

Glucose oxidase (GOX) enzyme can generate H2O2 by the oxidation of glucose in the presence of molecular oxygen. H2O2 has the potential to enhance biotic and abiotic stress tolerance of higher plants. The gene encoding glucose oxidase was isolated from the genomic DNA of Penicillium funiculosum. The GOX gene was cloned under the control of a CaMV 35S promoter into the plant transformation vector pBI121. Potato was transformed via an Agrobacterium tumefaciens-mediated procedure by selecting for kanamicyn resistance. Stable integration and expression of GOX gene in the transgenic potato lines were ascertained by molecular and biochemical tests. Transgene integration in the transformants was confirmed by PCR for GOX gene and multiplex PCR for GOХ gene, NOS-terminator, actin gene, VirE2 gene. Histochemical analyses for H2O2 production were studied using starch-KI medium. The transgenic plant lines will be used in further investigation.