Preprocessing of microscopy images via Shannon's entropy

Abstract

This document presents a novel method of image preprocessing based on Shannon's entropy. The method is developed a specially for microscopy images captured in phase-contrast mode. But it can be used in many others applications. Many variants of using entropy are described in the paper and differences are discussed. Performance of individual methods are illustrated. Finally, implementation on graphics cards to overpass higher computation requirements of the algorithm is described. The total speed-up of the processing is about 3600x.