Correction of experimental distortions in analysis of fluorescence intensity decays by the phase plane method

Abstract

In this paper we advance the phase plane method for analysis of fluorescence intensity decays to take into account the presence of such instrumental artifacts as time-uncorrelated background radiation and time shift between instrumental response function and sample fluorescence intensity decay. The developed non-iterative algorithm does not require prior selection of initial guesses and ensures accurate estimates for parameters. The results of simulation experiments are presented to demonstrate the applicability of the developed method.