Optical Methods for the Analysis of the Temoprofin Photosensitizer Distribution Between Serum Proteins and Methyl-β-Cyclodextrin Nanocarriers in Blood Serum

Abstract

Various optical methods for the analysis of the processes leading to temoporfin photosensitizer distribution between supramolecular nanosized inclusion complexes derived from β−cyclodextrins and blood serum proteins were examined. Methods involving induced circular dichroism, fluorescence anisotropy, and the variability of the shape of the photosensitizer fluorescence excitation spectra were compared with traditional methods such as gel chromatography and ultracentrifugation. The feasibility of using the photosensitizer optical characteristics for analyzing both equilibrium and kinetic processes of photosensitizer distribution in blood was demonstrated. The main advantages and limitations of these approaches in in vitro experiments were described.