Study of the effects of selenium nanoparticles and their combination with immunoglobulins on the survival and functional state of Polymorphonuclear cells

Abstract

Selenium and its compounds are promising immunomodulatory agents. We studied the ability of selenium nanoparticles (Se NPs) and their combination with immunoglobulins (IgG) to influence the functional responses of immune cells and the expression profile of “stress associated”. The cytotoxic effect of Se NPs was also studied in primary and immortalized cell cultures. Se NPs were obtained by laser ablation in water followed by fragmentation. Fragmentation control was performed using acoustic and optical methods of size estimation. The size distribution of Se NPs was narrow and an average size was 100 nm. The Se NPs did not exhibit cytotoxicity against fibroblasts, hepatocytes and cell line L-929. Weak cytotoxicity was found for the HL-60 granulocyte-like culture. On a surviving culture of mouse granulocytes, no cytotoxic effect was found. The addition of Se NPs in combination with IgG can modulate the maximum and total production of ROS by murine granulocytes induced by W-peptide and PMA and modulate the proportion of granulocytes with calcium responses to Wp. Se NPs modulate the action of IgG on the physiological responses of granulocytes. In the study of gene expression, similar patterns were found. Se NPs increase the expression of HSP90, NFkb, Xrcc4, SOD2 genes; IgG enhances the effect of Se NPs, while IgG decreased the expression of these genes. This phenomenon can be explained by the interaction between Se NPs and IgG. Data of spectral methods showed the binding of IgG to Se NP surface and a partial change in the spatial structure of IgG.