Влияние хронического гамма-облучения в дозе 0,5 Гр на кинетические параметры аденилатциклазы мозга крыс

Abstract

Chronic gamma irradiation (0,5 Gy) was shown to result in decrease of apparent maximal velocity of cAMP production by catalitic subunit, while Km value was unchanged in rat brain plasma membranes. The GTP causes a 2,2 fold and 4,2 fold increase in the apparent Vmax of catalitic subunit in control and irradiated animals, respectively. In the presence of GTP the apparent Km value was 3,2 times higher after irradiation than that in control. The inhibition of adenylate cyclase activity by high ATP concentrations, with or without GTP addition, was' more pronounced after irradiation than that in intact animals.