Logo BSU

Please use this identifier to cite or link to this item: https://elib.bsu.by/handle/123456789/21145
Title: Spectral Properties of Thioflavin T and Its Complexes with Amyloid Fibrils
Authors: Voropai, E. S.
Samtsov, M. P.
Kaplevskii, K. N.
Maskevich, A. A.
Stepuro, V. I.
Povarova, O. I.
Kuznetsova, I. M.
Turoverov, K. K.
Fink, A. L.
Uverskii, V. N.
Keywords: ЭБ БГУ::ЕСТЕСТВЕННЫЕ И ТОЧНЫЕ НАУКИ::Физика
Issue Date: 2003
Citation: Journal of Applied Spectroscopy.- 2003. - № 6. - С. 868-874.
Abstract: Comparative analysis of the absorption and fluorescence spectra and fluorescence excitation spectra of thioflavin T (ThT) in various solvents and in the composition of amyloid fibrils has shown that ThT, when excited in the region of the long-wavelength absorption band, fluoresces in the spectral region with a maximum at 478–484 nm. The appearance in aqueous and alcohol solutions of a fluorescence band with a maximum near 440 nm has been attributed to the presence in the composition of the ThT preparations of an impurity with an absorption band in the 340–350-nm range. The literature data showing that in glycerol ThT has a wide fluorescence spectrum with two maxima are due to the artifact connected with the use of a high concentration of the dye. It has been suggested that the cause of the low quantum yield of ThT aqueous and alcohol solutions is the breakage of the system of conjugated bonds due to the reorientation of the benzothiozole and benzaminic rings of ThT in the excited state with respect to one another. The main factor determining the high quantum yield of fluorescence of ThT incorporated in fibrils is the steric restriction of the rotation of the rings about one another under these conditions. The suggestions made have been verified by the quantum-chemical calculation of the ThT molecule geometry in the ground and excited states.
URI: http://elib.bsu.by/handle/123456789/21145
Appears in Collections:Статьи сотрудников НИИ ПФП

Files in This Item:
File Description SizeFormat 
Spectral Properties of Thioflavin T and Its Complexes with Amyloid Fibrils.pdf334,8 kBAdobe PDFView/Open


PlumX

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.