Влияние мутаций в генах peIW и kdgR на продукцию пектатлиаз у Erwinia carotovora subsp. atroseptica

Abstract

We have investigated the role of the regulatory protein KdgR in control of pectate lyase production in Erwinia carotovora subsp. atroseptica (Eca). The levels of extracellular pectate lyases and expression of the peIW gene coding for the cytoplasmic pectate lyase PeIW, are higher in the kdgR mutants than in kdgR* strains. The addition of polygalacturonic acid (universal inducer of pectate lyase genes) had no effect on the level of extracellular pectate lyases in the kdgR mutant. However, the level of extracellular pectate lyases production was lower in the presence of pectic compounds in strains with combined kdgR and pelW mutations. We propose that Eca bacteria, in contrast to Erwinia chrysanthemi, have an additional regulatory mechanism controlling the production of virulence factors which is sensitive to intermediate concentrations of pectin catabolism pathway.