Действие триазиновых гербицидов на активность АТФ-азы и пирофосфатазы тонопласта

Abstract

The effects of 3 triazine herbicides (atrazine, prometryne, simazine) on ATP- and PPi- dependent proton gradient development and ATP-hydrolysing activity by tonoplast-enriched vesicles isolated from leaves of Nicotiana tabacum plants were investigated. All triazine disrupted proton gradient formation across vesicles in both cases (ATP- and PPi-dependent) at the concentration higher than 0,1 mM. Haft-maximal inhibition concentration (I50) for these compounds were evaluated. However, triazine did not affect significantly the ATP-hydrolysing activity by to noplast vesicles at any concentration tested (0,02 - 1 mM). W e suggest that triazine herbicides inhibited tonoplast proton pumps indirectly by increasing passive proton leakage across membrane vesicles.