The Fluorescent Probing Method in the Estimation of the Functional Probabilities of the Blood Plasma Transport Systems

Abstract

During this work method of fluorescent probes with differently charged fluorescent dyes was used for estimation of binding ability of the blood plasma main transport proteins. Investigations were done at the patients with liver cirrhosis for creating on those basis fluorescent tests adapted for clinical use. Such tests should use for depending of severity degree of pathological process and for the correct choice of the most effective haemosorbent for extracorporeal blood purification in the definite clinical situation. Regularity was shown for the changes in the binding ability of the blood plasma main transport proteins in liver cirrhosis, which depended on the stage of the disease and allowed efficiently estimate severity degree of pathological process. Comparative evaluation of the efficacy of different charcoal haemosorbents widely used in clinical practice as well as "Prometheus" device was done in terms of endogenous intoxication syndrome correction at liver cirrhosis patients using results of biochemical analysis and method of fluorescent dyes. It was shown that the most effective sorbents were sorbents of "Prometheus" and between charcoal heamosorbents - "Carbocol".