Potential of using cultivated dermal fibroblasts on the biodegradable polymeric matrices for treating skin damages in the experiment

Abstract

Objectives. To evaluate the possibility of using of cultivated dermal fibroblasts on the biodegradable polymeric matrices for treating skin damages in the experiment. Methods. The study was performed on 90 rats of Wistar line (180-200 g weight) with pure wounds (D-2,5cm). Six groups were formed, 15 animals per each group. The culture of dermal fibroblasts was isolated from the skin of newborn rats; to accumulate the required biomass of cells subpassage was carried out. The cellular biological products were not applied in group 1 (control). Biodegradable matrices (dextran phosphate or polylactide) were used for treating wounds in the groups 2-6 as matrices without cellular biological product and matrices with cultured fibroblasts (250×103 to 700×103 cells per wound). Conclusion about the impact of biodegradable polymeric matrices with dermal fibroblasts on the wound healing was made on the basis of a comparative analysis of the results of digital planimetry and morphological studies. Results. At all stages (7, 14, 21, 28 days) minimal rate of healing was registered in the first group. The sixth group, where polylactide containing 700×103 cells was added in the wound, is characterized by the biggest values of the healing rate - with respect to other groups. Complete epithelization of the wound defects in the micropreparations of the sixth group was marked by the 21st day of the experiment. The strong direct correlation between the number of transplanted cells and wound rate healing was detected on the 7th day (ρ=0,923), the 14th day (ρ=0,924), and the 21st day of the experiment (ρ=0,914). Conclusion. In the experiment it has been found out, that local application of the cultivated dermal fibroblasts on biodegradable polymer matrices (dextran phosphate and polylactide) results in a significant reduction of terms of the wound epithelization.