Акусто-ферментативный тромболизис: влияние ультразвуковой обработки на молекулярные свойства стрептокиназы и автоактивацию предшественников сериновых протеиназ

Abstract

The continuous ultrasonic (US) processing within 10 minutes is not accompanied by change of structural and functional properties of streptokinase. The increase of continuous US-treatment (10-60 minutes) is accompanied by disturbance of structural organization of streptokinase and irreversible partial aggregation of protein. Ultrasound modified streptokinase promotes an autoactivation of plasminogen. Simultaneously in a streptokinase molecule additional site accessible for proteolytic cleavage by a plasmin appeared. The increase of an exponential phase and decreasing of the rate of zymogens autoactivation process at US-processing is revealed. From four proteins (chymotrypsinogen, trypsinogen, chymotrypsin and trypsin), participating in a proteolytic autoactivation, most sensitive to the US treatment is trypsin.